![]() AtProRS-Cyt mRNA is more highly expressed in roots than in cotyledons. AtProRS-Org (At5g52520) is localized in chloroplasts/mitochondria, and AtProRS-Cyt (At3g62120) is cytosolic. The growth of Arabidopsis thaliana seedling roots is more sensitive to inhibition by A 2C than is cotyledon growth. ![]() The toxicity of azetidine- 2- carboxylic acid (A 2C), a structural analogue of L-proline, results from its incorporation into proteins due to misrecognition by prolyl-tRNA synthetase (ProRS). ![]() Lee, Jiyeon Joshi, Naveen Pasini, Rita Dobson, Renwick C J Allison, Jane Leustek, Thomas Inhibition of Arabidopsis growth by the allelopathic compound azetidine- 2- carboxylate is due to the low amino acid specificity of cytosolic prolyl-tRNA synthetase. Six new azetidin- 2-ones of ferulic acid were synthesized, physicochemically characterized and validated spectrally. They were characterized in terms of their physicochemical properties and their structure was confirmed by IR and 1H-NMR spectroscopy. Six new azetidin- 2-ones of ferulic acid were synthesized. The synthesis was carried out in several steps: (i) obtaining the ferulic acid chloride (ii) obtaining the ferulic acid hydrazide with hydrazine hydrate (98%) (iii) condensation of ferulic acid hydrazide with different benzaldehydes ( 2-hydroxy-/ 2-nitro-/4-chloro-/4- fluoro-/4-bromo-benzaldehyde) in order to obtain the corresponding hydrazones (iv) cy- clization of ferulic acid hydrazones with chloroacethyl chloride in freshly distilled toluene medium and in the presence of triethylamine, resulting in the corresponding azetidin- 2-ones. To synthesize some new azetidin- 2-ones of ferulic acid and to evaluate them from physicochemical and spectral point of view. Stan, Cătălina Daniela Drăgan, Maria Pânzariu, Andreea Profire, LenuÅ£a cerevisiae.ĮVALUATION OF THE SYNTHESIS AND STRUCTURE OF NEW AZETIDIN- 2-ONES OF FERULIC ACID. cerevisiae strains, all of the recombinants were resistant to L-azetidine- 2- carboxylic acid, indicating that both MPR1 and MPR 2 are expressed and have a global function in S. When this novel MPR1 or MPR 2 gene (sigma 1278b gene for L-proline analogue resistance) was introduced into the other S. Genomic Southern analysis and gene disruption showed that two copies of the novel gene with one amino acid change at position 85 required for L-azetidine- 2- carboxylic acid resistance were present on chromosomes X and XIV of Sigma 1278b background strains. The protein sequence was found to be a member of the N-acetyltransferase superfamily. Deletion analysis indicated that one open reading frame encoding a predicted protein of 229 amino acids is indispensable for L-azetidine- 2- carboxylic acid resistance. The nucleotide sequence of a 4.5-kb segment exhibited no identity with the sequence in the genome project involving strain S288C. cerevisiae strains S288C and Sigma 1278b. The 5.4 kb-DNA fragment was cloned from the genomic library of the L-azetidine- 2- carboxylic acid-resistant mutant derived from a cross between S. We discovered on the chromosome of Saccharomyces cerevisiae Sigma 1278b novel genes involved in L-proline analogue L-azetidine- 2- carboxylic acid resistance which are not present in the standard laboratory strains. Takagi, H Shichiri, M Takemura, M Mohri, M Nakamori, S Saccharomyces cerevisiae sigma 1278b has novel genes of the N-acetyltransferase gene superfamily required for L-proline analogue resistance.
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